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FACTOR VIII ACTIVITY, CLOTTING

Test Number:
  • 0030095
Test Alias:
Preferred Specimen:
  • Plasma
Container Type:
  • 3.2% sodium citrate (light blue-top) tube
Alternative Specimen:
Volume:
  • 2 mL
Collection Instructions:
  • Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately and place in specimen bag with "Frozen Specimen" label applied and transport frozen. Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII and von Willebrand Factor studies.
Transport Temperature:
  • Frozen
Specimen Stability:
  • Room temperature: Unacceptable
  • Refrigerated: Unacceptable
  • Frozen -20° C: 14 days
  • Frozen -70° C: 1 year
Methodology:
  • Clotting
CPT Code:
  • 85240
Reject Criteria:
  • Hemolysis • Received room temperature • Received refrigerated • Received thawed
Reference Range:
  • 50-180 % normal
Report Available:
  • 6 days

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